NCS1 antibody (AA 1-50)

Catalog No. ABIN1385755

This Rabbit Polyclonal antibody specifically detects NCS1 in WB, ELISA, IF (p), IHC (p), IF (cc), IHC (fro) and ICC. It exhibits reactivity toward Rat.

Quick Overview for NCS1 antibody (AA 1-50) (ABIN1385755)

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-NCS1 Antibody

Binding Specificity: AA 1-50

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NCS1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NCS1

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Alternative Name: NCS1/Frequenin

Background:

Synonyms: 9430075O15Rik, A730032G13Rik, AI836659, DKFZp761L1223, FLUP, Frequenin, Frequenin homolog Drosophila, Frequenin homolog, Frequenin like protein, Frequenin, Drosophila, homolog of, Frequenin-like protein, Frequenin-like ubiquitous protein, Mfreq, NCS 1, NCS-1, ncs1, NCS1_HUMAN, Neuronal calcium sensor 1, Neuronal Calicum Sensor 1.

Background: NCS-1 (for neuronal calcium sensor-1, also designated frequenin) belongs to a highly conserved family of EF-hand-containing Ca++-binding proteins expressed mainly in neurons. NCS-1 is localized to neuronal cell bodies and axons throughout the brain and spinal cord. It is also expressed in glial cells and in neuroendocrine bovine adrenal chromaffin and PC12 cells. NCS-1 is a regulatory protein involved in Ca++-dependent exocytosis of synaptic vesicles and dense core granules. NCS-1 also functions in the voltage-independent autocrine pathway that negatively regulates non-L-type Ca++ channels.