SPEF2 antibody (AA 227-275)

Catalog No. ABIN1386162

The Rabbit Polyclonal anti-SPEF2 antibody (ABIN1386162) specifically detects SPEF2 in WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) and ICC. The antibody is reactive with Human samples.

Quick Overview for SPEF2 antibody (AA 227-275) (ABIN1386162)

Target: SPEF2 (Sperm Flagellar 2 (SPEF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPEF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-SPEF2 Antibody

Binding Specificity: AA 227-275

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human KPL2/Sperm Flagellar 2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SPEF2

Target: SPEF2 (Sperm Flagellar 2 (SPEF2))

Alternative Name: KPL2/Sperm Flagellar 2

Background:

Synonyms: KPL2, MGC102842, Protein KPL2, SPEF2, sperm flagellar protein 2, FLJ23164, FLJ23577, FLJ25395, KIAA1770, SPEF2_HUMAN.

Background: Flagella and cillia are both membrane-bound projections from the cell surface that beat in distinctive patterns. Cilia are shorter and usually more profuse than flagella and contain a microtubule cytoskeleton, the ciliary axoneme, surrounded by a ciliary membrane. The ciliary membranes of all cilia hold specific receptors and ion channel proteins that initiate signaling pathways that regulate motility and/or link mechanical or chemical stimuli to intracellular transduction cascades regulating differentiation, migration and cell growth during development and in adulthood. KPL2, also known as SPEF2 (sperm flagellar 2), is a 1,822 amino acid protein that contains a calponin homology domain, three nuclear localization signals, a consensus P-loop and a proline-rich region. Required for correct axoneme develoment, KPL2 is predominantly expressed in cells with cilia or flagella. Four isoforms of KPL2 exists as a result of alternative splicing events.