DPF1 antibody (AA 301-380)

Catalog No. ABIN1386170

The Rabbit Polyclonal anti-DPF1 antibody is suitable to detect DPF1 in samples from Mouse and Rat. It has been validated for WB, ELISA, ICC, IF (cc), IF (p), IHC (fro) and IHC (p).

Quick Overview for DPF1 antibody (AA 301-380) (ABIN1386170)

Target: DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DPF1 Antibody

Binding Specificity: AA 301-380

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Neuro D4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DPF1

Target: DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))

Alternative Name: Neuro D4/DPF1

Background:

Synonyms: NeuroD4, BAF45B, BRG1-associated factor 45B, D4, DPF1, DPF 1, DPF=1, DPF1_HUMAN, NEUD4, NEUD 4, Zinc and double PHD fingers family 1, Zinc finger protein neuro-d4.

Background: DPF1 is a 353 amino acid protein that contains two PHD-type zinc fingers and belongs to the requiem/DPF family. Localized to both the nucleus and the cytoplasm, DPF1 is thought to play an important role in the regulation of neuronal cell survival. Specifically, DPF1 may function as a neurospecific transcription factor that binds DNA and participates in cell cycle progression. Human and rat DPF1 share 93 % sequence identity, suggesting a conserved role between species. Multiple isoforms of DPF1 exist due to alternative splicing events.