Tppp antibody (AA 61-150)

Catalog No. ABIN1386264

This anti-Tppp antibody is a Rabbit Polyclonal antibody detecting Tppp in WB, ELISA, IF (cc), IF (p), ICC, IHC (p) and IHC (fro). Suitable for Mouse.

Quick Overview for Tppp antibody (AA 61-150) (ABIN1386264)

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tppp antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Tppp Antibody

Binding Specificity: AA 61-150

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TPPP

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Tppp

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Alternative Name: TPPP

Background:

Synonyms: Tubulin Polymerization Promoting Protein 24, 25 kDa brain specic protein, 25 kDa brain-specic protein, Brain specic protein p25 alpha, Glycogen synthase kinase 3 GSK3 inhibitor p24, p24, p25, p25-alpha, p25alpha, TPPP, TPPP/p25, TPPP_HUMAN, TPPP1, Tubulin polymerization promoting protein, Tubulin polymerization-promoting protein.

Background: Tubulin family members are globular proteins important in the assembly of microtubules. Microtubules are structural components that play important roles in mitosis, cytokinesis and vesicle transport. TPPP (Tubulin polymerization-promoting protein), also known as p24 and p25, is a widely expressed 219 amino acid protein found in the perinuclear region of the cytoplasm. TPPP may form dimers and functions in polymerizing tubulin into double-walled tubules, polymorphic aggregates, or stabilized blocks. TPPP overexpression prevents formation of the mitotic spindle assembly and breakdown of the nuclear envelope. TPPP is phosphorylated by TPK II and is encoded by a gene that maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6 % of the human genome.