WT1 antibody (AA 301-400)

Catalog No. ABIN1386293

The Rabbit Polyclonal anti-WT1 antibody has been validated for FACS, WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect WT1 in samples from Human, Rat, Mouse and Cow. There is 1 publication available.

Quick Overview for WT1 antibody (AA 301-400) (ABIN1386293)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human, Rat, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-WT1 Antibody

Binding Specificity: AA 301-400

Cross-Reactivity: Cow, Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Wilms Tumor Protein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-WT1 antibody (ABIN1386293)

Xiao, Guan, Nie, Wang, Sun, He, Huang, Zhang, Yang, Wang, Zhao: "Rapamycin promotes podocyte autophagy and ameliorates renal injury in diabetic mice." in: Molecular and cellular biochemistry, Vol. 394, Issue 1-2, pp. 145-54, (2014) (PubMed).

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: Wilms Tumor Protein

Background:

Synonyms: GUD, AWT1, WAGR, WT33, NPHS4, WIT-2, EWS-WT1, Wilms tumor protein, WT1

Background: Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.

Molecular Weight: 55kDa

Gene ID: 7490

UniProt: P19544

Pathways: Tube Formation