NME4 antibody (AA 21-120)

Catalog No. ABIN1386601

This Rabbit Polyclonal antibody specifically detects NME4 in WB, ELISA, IHC (p), IF (cc), IF (p), ICC and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for NME4 antibody (AA 21-120) (ABIN1386601)

Target: NME4 (NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NME4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NME4 Antibody

Binding Specificity: AA 21-120

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NME4/nm23-H4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NME4

Target: NME4 (NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4))

Alternative Name: NME4/nm23-H4

Background:

Synonyms: mitochondrial, NDK, NDKM_HUMAN, NDP kinase, NDP kinase, mitochondrial, NDPKD, nm23 H4, nm23-H4, NM23D, Nm23M4, NME4, Non metastatic cells 4 protein expressed in, Nucleoside diphosphate kinase D, Nucleoside diphosphate kinase, mitochondrial.

Background: The nm23 gene (Metastasis inhibition factor nm23), a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. Based on sequence analysis, nm23, also designated nucleoside diphosphate kinase A (NDK A) or Tumor metastatic process-associated protein, appears to be highly related to nucleotide diphosphate kinases (NDP). NDP kinases A and B are identical to two isotypes of human nm23 homologs, nm23-H1 and nm23-H2, respectively. nm23-H2 is also identical in sequence to PuF, a transcription factor that binds to nuclease-hypersensitive elements at positions 142 to 115 of the human c-Myc promoter. nm23-H3 and nm23-H4 are important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. nm23-H4 localizes to the mitochondrial intermembrane space and is widely expressed, with higher levels detected in prostate, heart, liver, small intestine, and skeletal muscle tissues. Low amounts of nm23-H4 are observed in the brain and in blood leukocytes.

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process