KDM4B antibody (pThr305)

Catalog No. ABIN1386741

This anti-KDM4B antibody is a Rabbit Polyclonal antibody detecting KDM4B in WB, ELISA, ICC, IHC (p), IF (cc), IF (p) and IHC (fro). Suitable for Mouse and Rat.

Quick Overview for KDM4B antibody (pThr305) (ABIN1386741)

Target: KDM4B (Lysine (K)-Specific Demethylase 4B (KDM4B))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM4B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-KDM4B Antibody

Binding Specificity: pThr305

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human JMJD2B around the phosphorylation site of Thr305

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for KDM4B

Target: KDM4B (Lysine (K)-Specific Demethylase 4B (KDM4B))

Alternative Name: JMJD2B

Background:

Synonyms: KDM4B/JMJD2B phospho T305, KDM4B/JMJD2B phospho Thr305, p-KDM4BT305, p-KDM4BThr305, JHDM3B, JmjC domain-containing histone demethylation protein 3B, JMJD2B, JmjC domain-containing histone demethylation protein 3A, Jumonji domain containing protein 2B, KIAA0876, Lysine-specic demethylase 4B, KDM4B_HUMAN.

Background: JMJD2B is a 1,064 amino acid protein encoded by the human gene JMJD2B. JMJD2B belongs to the JMJD2B histone demethylase family and contains one JmjC domain, one JmjN domain, two PHD-type zinc fingers and two Tudor domains. The two Tudor domains recognize and bind methylated histones and have an interdigitated structure, the unusual fold is required for its ability to bind methylated histone tails. JMJD2B is a histone demethylase that specifically demethylates Lys 9 residues of Histone H3, thereby playing a role in histone code. It does not demethylate Histone H3 Lys 4, H3 Lys 27, H3 Lys 36 or H4 Lys 20, however, and is only able to demethylate trimethylated H3 Lys-9 and has weaker activity than JMJD2A, JMJD2C and JMJD2D. JMJD2B demethyl-ation of Lysine residues will generate formaldehyde and succinate. JMJD2B is a ubiquitously expressed nuclear protein.

Gene ID: 23030

Pathways: Warburg Effect