ZNF148 antibody (AA 303-380)

Catalog No. ABIN1386746

The Rabbit Polyclonal anti-ZNF148 antibody has been validated for WB, ELISA, ICC, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect ZNF148 in samples from Human.

Quick Overview for ZNF148 antibody (AA 303-380) (ABIN1386746)

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF148 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ZNF148 Antibody

Binding Specificity: AA 303-380

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ZNF148/ZBP-89

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ZNF148

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Alternative Name: ZNF148/ZBP-89

Background:

Synonyms: Transcription factor ZBP-89, Transcription factor ZBP89, ZBP89, Zinc finger DNA binding protein 89, Zinc finger DNA-binding protein 89, Zinc finger protein 148, ZN148_HUMAN, ZNF 148, ZNF148.

Background: ZNF148 is a zinc finger transcription factor that is universally expressed. ZBP-89, a Kruppel-like repressor protein, is the silencer element binding factor for Vimentin. ZBP-89 has been shown to bind to GC-rich DNA elements in promoters for gastrin, ornithine decarboxylase and the cyclin-dependent kinase inhibitor p21 (also designated Cip1 or WAF1). ZBP-89 expression is induced by trans-retinoic acid or butyrate, which also induces terminal differentiation of colon cancer cells. ZBP-89 cooperates with histone acetyltransferase coactivator p300 in the regulation of p21, a cyclin-dependent kinase inhibitor whose associated gene is a target gene of p53. ZBP-89 also regulates cell proliferation, in part, through its ability to directly bind the p53 protein and retard its nuclear export. Elevated levels of ZBP-89 induce growth arrest and apoptosis in human gastrointestinal cells.

Gene ID: 7707