MARCH4 antibody

Catalog No. ABIN1387034

The Rabbit Polyclonal anti-MARCH4 antibody has been validated for WB, IF (p) and IHC (p). It is suitable to detect MARCH4 in samples from Human, Mouse and Rat.

Quick Overview for MARCH4 antibody (ABIN1387034)

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MARCH4 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MARCH4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MARCH4

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Alternative Name: MARCH4

Background:

Synonyms: E3 ubiquitin protein ligase MARCH4, RNF174, KIAA1399, MARCH 4, MARCH IV, Membrane associated RING CH protein IV, Membrane associated ring finger C3HC4 4, Membrane associated RING finger protein 4, mKIAA1399, RING finger protein 174, MARH4_HUMAN.

Background: MARCH4, an E3 ubiquitin protein ligase, may mediate ubiquitination of MHC I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin protein ligases accept ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Gene ID: 57574