MARCH7 antibody (AA 65-115)

Catalog No. ABIN1387036

Product Details anti-MARCH7 Antibody

Target: MARCH7 (Membrane-Associated Ring Finger (C3HC4) 7, E3 Ubiquitin Protein Ligase (MARCH7))

Binding Specificity: AA 65-115

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MARCH7

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IP(1-2 μg)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-MARCH7 antibody (ABIN1387036)

Zhang, Wang, Tian, Li: "Expression of USP7 and MARCH7 Is Correlated with Poor Prognosis in Epithelial Ovarian Cancer." in: The Tohoku journal of experimental medicine, Vol. 239, Issue 3, pp. 165-75, (2016) (PubMed).

Hu, Meng, Yu, Hu, Mao: "Ubiquitin E3 ligase MARCH7 promotes ovarian tumor growth." in: Oncotarget, Vol. 6, Issue 14, pp. 12174-87, (2015) (PubMed).

Target Details for MARCH7

Target: MARCH7 (Membrane-Associated Ring Finger (C3HC4) 7, E3 Ubiquitin Protein Ligase (MARCH7))

Alternative Name: MARCH7 (MARCH7 Products)

Background:

Synonyms: AXO, AXOT, Axotrophin, E3 ubiquitin-protein ligase MARCH7, MARCH VII, MARCH-VII, March7, MARH7_HUMAN, Membrane associated RING CH protein VII, Membrane associated ring finger C3HC4 7 E3 ubiquitin protein ligase, membrane associated ring finger C3HC4 7, Membrane associated RING finger protein 7, Membrane-associated RING finger protein 7, Membrane-associated RING-CH protein VII, RING finger protein 177, RNF177.

Background: Axotrophin is a stem cell gene that encodes a protein which is involved in T lymphocyte regulation (especially in regulating the proliferation) and leukemia inhibitory factor (LIF) release. LIF is a neuropoietic cytokine that is important for stem cell regulation and thymocyte stimulation. Both Axotrophin and LIF are linked to transplantation intolerance. Axotrophin is also involved in corpus callosum differentiation and may play a role in glial cell line-derived neurotrophic factor (GDNF)-dependent sensory neuron survival in the substantia gelatinosa of the adult spinal cord. Axotrophin is primarily expressed in the hippocampus, cortex, purkinje and granule cells of the cerebellum.

Gene ID: 64844

UniProt: Q9H992