ODF3 antibody

Catalog No. ABIN1387063

This Rabbit Polyclonal antibody specifically detects ODF3 in WB, IHC (p) and IF (p). It exhibits reactivity toward Mouse, Human and Rat.

Quick Overview for ODF3 antibody (ABIN1387063)

Target: ODF3 (Outer Dense Fiber of Sperm Tails 3 (ODF3))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ODF3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ODF3 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ODF3/CT135

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ODF3

Target: ODF3 (Outer Dense Fiber of Sperm Tails 3 (ODF3))

Alternative Name: CT135

Background:

Synonyms: Cancer/testis antigen 135, CT135, hSHIPPO, odf3, ODF3A_HUMAN, Outer dense fiber of sperm tails 3, Outer dense fiber of sperm tails protein 3, Outer dense fiber protein 3, SHIPPO1, Sperm tail protein SHIPPO 1, Sperm tail protein SHIPPO1, TISP50, Transcript induced in spermiogenesis protein 50.

Background: Outer dense fibers are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail. May help to maintain the passive elastic structures and elastic recoil of the sperm tail.Constituting the main cytoskeletal structure of spermatid flagella, outer dense fibers (ODFs) add elastic recoil, stiffness and protection against shear forces during sperm movement. Human ODFs consist of approximately 10 major and at least 15 minor proteins. The major proteins of the ODF include Odf1, Odf2, and Odf3, which compose a family of proteins that are preferentially expressed during mammalian spermiogenesis. Odf3 (outer dense fiber protein 3), also known as sperm tail protein SHIPPO 1 and TISP50 (transcript induced in spermiogenesis protein 50), is a 254 amino acid protein that is expressed during the latter part of spermatogenesis in flagella of elongated spermatids and mature sperm. Odf proteins are directed to their exact subcellular location by Spags, which are characterized as chaperone-like Odf-binding molecules. There are two isoforms of Odf3 that are produced as a result of alternative splicing events.

Gene ID: 113746