B-Cell Linker antibody (pTyr189)

Catalog No. ABIN1387122

This anti-B-Cell Linker antibody is a Rabbit Polyclonal antibody detecting B-Cell Linker in WB, IF (p) and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for B-Cell Linker antibody (pTyr189) (ABIN1387122)

Target: B-Cell Linker (BLNK)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B-Cell Linker antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-B-Cell Linker Antibody

Binding Specificity: pTyr189

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human BLNK around the phosphorylation site of Tyr189 [EN(p-Y)IH]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK

Background:

Synonyms: BLNK phospho Y491, p-BLNK phospho Y491, B cell adapter containing SH2 domain protein, B cell adapter containing Src homology 2 domain protein, B cell linker, B cell linker protein, B cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, B-cell linker protein, BASH, BASH, Bca, Bca, BLNK, BLNK s, BLNK_HUMAN, Cytoplasmic adapter protein, Ly 57, Ly-57, Ly57, Lymphocyte antigen 57, Lymphocyte antigen-57, Lyw 57, Lyw-57, Lyw57, MGC111051, SLP 65, SLP65, SLP-65, Src homology 2 domain containing leukocyte protein of 65 kDa.

Background: This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012].

Gene ID: 29760

Pathways: BCR Signaling