E4F1 antibody

Catalog No. ABIN1387131

The Rabbit Polyclonal anti-E4F1 antibody (ABIN1387131) specifically detects E4F1 in WB, IHC (p) and IF (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for E4F1 antibody (ABIN1387131)

Target: E4F1 (E4F Transcription Factor 1 (E4F1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E4F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-E4F1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human E4F1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for E4F1

Target: E4F1 (E4F Transcription Factor 1 (E4F1))

Alternative Name: E4F1

Background:

Synonyms: E4F, E4F transcription factor 1, isoform CRA_b, P120E4F transcription factor, E4F1_HUMAN.

Background: E4F1, also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains nine C2H2-type zinc fingers. Expressed ubiquitously in adult and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control. Additionally, E4F1 is thought to function as a ubiquitin ligase that mediates the ubiquitination (and subsequent degradation) of target proteins and may be involved in the p53 tumor suppressor pathway. E4F1, which may be post-translationally phosphorylated or sumoylated, is subject to proteolytic cleavage which results in the creation of a short peptide with specific DNA binding capabilities.

Gene ID: 1877