UCHL3 antibody (AA 133-230)

Catalog No. ABIN1387243

This Rabbit Polyclonal antibody specifically detects UCHL3 in WB, ELISA, IHC (p), ICC, IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human and Ratand has been mentioned in 1 publication.

Quick Overview for UCHL3 antibody (AA 133-230) (ABIN1387243)

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-UCHL3 Antibody

Binding Specificity: AA 133-230

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UCHL3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-UCHL3 antibody (ABIN1387243)

Wang, Yu, Hu, Cheng, Li: "Ubiquitin Carboxy-Terminal HydrolaseL3 Correlates with Human Sperm Count, Motility and Fertilization." in: PLoS ONE, Vol. 11, Issue 10, pp. e0165198, (2016) (PubMed).

Target Details for UCHL3

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Alternative Name: UCHL3

Background:

Synonyms: Ubiquitin carboxyl-terminal hydrolase isozyme L3, Ubiquitin thioesterase L3, Ubiquitin thiolesterase, Ubiquitin thiolesterase L3, UCH L3, UCH-L3, UCHL3, UCHL3_HUMAN.

Background: UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. UCH-L1 is expressed in brain neurons. Examination of specific brain regions reveals expression in all areas tested, particularly in the substantia nigra. UCH-L1 represents 1 to 2 % of total soluble brain protein. Its occurrence in Lewy bodies and its function in the proteasome pathway make it a compelling candidate gene in Parkinson disease. The gene which encodes UCH-L1 maps to human chromosome 4p14. The 230 amino acid human UCH-L3 protein is 54 % identical to that of UCH-L1. UCH-L3 is the predominant thiol protease and has high-affinity binding sites for ubiquitin.

Gene ID: 7347

UniProt: P15374

Pathways: Feeding Behaviour, Positive Regulation of fat Cell Differentiation