Peroxiredoxin 2 antibody (AA 131-198)

Catalog No. ABIN1387547

The Rabbit Polyclonal anti-Peroxiredoxin 2 antibody has been validated for ELISA, ICC, IHC (p), FACS, IF (cc), IHC (fro) and IF (p). It is suitable to detect Peroxiredoxin 2 in samples from Human and Rat.

Quick Overview for Peroxiredoxin 2 antibody (AA 131-198) (ABIN1387547)

Target: Peroxiredoxin 2 (PRDX2)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Peroxiredoxin 2 antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Peroxiredoxin 2 Antibody

Binding Specificity: AA 131-198

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Peroxiredoxin-2

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Peroxiredoxin 2

Target: Peroxiredoxin 2 (PRDX2)

Alternative Name: Peroxiredoxin 2

Background:

Synonyms: MGC4104, Natural killer cell enhancing factor B, Natural killer cell-enhancing factor B, Natural Killer Enhancing Factor B, NKEF B, NKEF-B, NKEFB, Peroxiredoxin-2, PRDX 2, PRDX2, PRDX2_HUMAN, PRP, PRX2, PRXII, TDPX1, Thiol Specic Antioxidant 1, Thiol specic antioxidant protein, Thiol-specic antioxidant protein, Thioredoxin Dependent Peroxide Reductase 1, Thioredoxin Peroxidase 1, Thioredoxin-dependent peroxide reductase 1, Torin, TPX1, TSA.

Background: Peroxiredoxin (Prx) is an antioxidant enzyme detoxifying reactive oxygen species and has a cysteine at the active site. Prx enzymes modulate various receptor signaling pathways and protect cells from oxidatively induced death. Peroxiredoxin 1 to 4 have two conserved Cys residues corresponding to Cys51 and Cys172 of mammalian Peroxiredoxin 1. The active site cysteine(Cys51) is oxidized to cysteine sulfenic acid(Cys51-SOH) when a peroxide is reduced. Because Cys51-SOH is unstable, it forms a disulfide with Cys172-SH which comes from the other subunit of the homodimer. The disulfide is then reduced back to the Prx active thiol form by the thioredoxin-thioredoxin reductase system. However, the formation of the disulfide is a slow process. Thus under oxidative stress conditions, the sulfenic intermediate(Cys51-SOH) can be easily over oxidized to cysteine sulfinic acid(Cys-SO2H) or cysteine sulfonic acid(Cys-SO3H) before it is able to form a disulfide. Recent studies suggest that over oxidized Prx can be reduced back to the active form during recovery after oxidative stress.

Gene ID: 7001