GPR147 antibody (AA 151-260)

Catalog No. ABIN1387655

The Rabbit Polyclonal anti-GPR147 antibody has been validated for ELISA, ICC, IF (cc), IF (p), IHC (fro), IHC (p) and WB. It is suitable to detect GPR147 in samples from Human, Mouse and Rat.

Quick Overview for GPR147 antibody (AA 151-260) (ABIN1387655)

Target: GPR147 (G Protein-Coupled Receptor 147 (GPR147))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details anti-GPR147 Antibody

Binding Specificity: AA 151-260

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GPR147/NPFF1 Receptor

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GPR147

Target: GPR147 (G Protein-Coupled Receptor 147 (GPR147))

Alternative Name: GPR147/NPFF1 Receptor

Background:

Synonyms: G Protein Coupled Receptor 147, G-protein coupled receptor 147, GPR147, Neuropeptide FF receptor 1, NPFF1, NPFF1_HUMAN, NPFF1R1, NPFFR1, RFamide related peptide receptor OT7T022, RFamide-related peptide receptor OT7T022.

Background: Neuropeptide FF 1 Receptor (NPFF1 or hFF1) and Neuropeptide FF 2 Receptor (NPFF2) belong to the G protein-coupled receptor 1 family. Both NPFF1 and NPFF2 are integral membrane proteins that act as receptors for NPAF (A-18- F-amide) and NPFF (F-8-F-amide) neuropeptides. Both NPFF proteins may be activated by synthetic or naturally occurring FMRF-amide-like ligands. The receptors are mediated by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. NPFF1 Receptors is highly expressed in the human hypothalamus and amygdala, indicating a possible role for NPFF1 in central autonomic and neuroendocrine control in the human brain. Based in part on NPFF2 Receptor expression in diencephalon and superficial layers of the spinal cord, NPFF2 Receptor is thought to be involved in the modulation of sensory input and opioid analgesia.