PRDM1 antibody (AA 501-600)

Catalog No. ABIN1387860

This anti-PRDM1 antibody is a Rabbit Polyclonal antibody detecting PRDM1 in WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). Suitable for Mouse.

Quick Overview for PRDM1 antibody (AA 501-600) (ABIN1387860)

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRDM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PRDM1 Antibody

Binding Specificity: AA 501-600

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PRDM1/Blimp1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PRDM1

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Alternative Name: PRDM1

Background:

Synonyms: Blimp 1, Blimp-1, B Lymphocyte Induced Maturation Protein 1, Beta interferon gene positive regulatory domain I binding factor, Beta-interferon gene positive regulatory domain I-binding factor, BLIMP-1, BLIMP1, Positive Regulatory Domain I Binding Factor 1, Positive regulatory domain I-binding factor 1, PR Domain Containing 1, PR domain containing 1 with ZNF domain, PR domain containing 1 with ZNF domain isoform 2, PR domain containing protein 1, PR domain zinc finger protein 1, PR domain-containing protein 1, PRDI BF1, PRDI binding factor 1, PRDI-BF1, PRDI-binding factor 1, PRDM 1, Prdm1, PRDM1_HUMAN.

Background: The development and differentiation of plasma cells, which are terminally differentiated B-cells, are induced by Blimp-1 (B lymphocyte-induced maturation protein, also designated PRDI-BF1). Blimp-1 is a transcriptional repressor that localizes to the nucleus and is considered a master regulator of terminal B-cell development. Alone, Blimp-1 is sufficient to trigger terminal B-cell differentiation. Blimp-1 upregulates the expression of syndecan-1 and J chain, represses IFN-b gene transcription and associates with HDAC to recruit it to DNA, thereby repressing c-myc. Blimp-1 is expressed during the late stages of B-cell differentiation in immunoglobulin-secreting plasma cells, as well as in long-lived, bone marrow plasma cells. The expression of Blimp-1 defines a checkpoint beyond which fully activated B cells proceed to the plasma cell stage, whereas immature and partially activated cells are eliminated.

Gene ID: 144423

Pathways: Regulation of Muscle Cell Differentiation