CH25H antibody (AA 1-100)

Catalog No. ABIN1387938

This anti-CH25H antibody is a Rabbit Polyclonal antibody detecting CH25H in WB. Suitable for Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for CH25H antibody (AA 1-100) (ABIN1387938)

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CH25H antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CH25H Antibody

Binding Specificity: AA 1-100

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CH25H

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CH25H antibody (ABIN1387938)

Mukherjee, Hough, Chattopadhyay, Navab, Fogelman, Meriwether, Williams, Bensinger, Moller, Faull, Lusis, Iruela-Arispe, Bostrom, Tontonoz, Reddy, Fogelman: "Transgenic tomatoes expressing the 6F peptide and ezetimibe prevent diet-induced increases of IFN-β and cholesterol 25-hydroxylase in jejunum." in: Journal of lipid research, Vol. 58, Issue 8, pp. 1636-1647, (2017) (PubMed).

Target Details for CH25H

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Alternative Name: CH25H

Background:

Synonyms: C25H, Cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, h25OH, CH25H

Background: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

Gene ID: 9023

UniProt: O95992