CD8 alpha antibody (AA 51-150)

Catalog No. ABIN1387948

This Rabbit Polyclonal antibody specifically detects CD8 alpha in FACS, WB, ELISA, IHC (fro), IHC (p), IF (p) and IF (cc). It exhibits reactivity toward Human, Rat and Chicken and has been mentioned in 2+ publications.

Quick Overview for CD8 alpha antibody (AA 51-150) (ABIN1387948)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Human, Rat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-CD8 alpha Antibody

Binding Specificity: AA 51-150

Cross-Reactivity: Chicken, Human, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat CD8 alpha

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD8 alpha antibody (ABIN1387948)

Savvatis, Müller, Fröhlich, Pappritz, Zietsch, Hamdani, Grote, Schieffer, Klingel, Van Linthout, Linke, Schultheiss, Tschöpe: "Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis." in: Basic research in cardiology, Vol. 109, Issue 6, pp. 449, (2014) (PubMed).

Cheng, Guo, Qiao, Liu, Nie, Li, Wang, Jiang: "T cell immunohistochemistry refines lung transplant acute rejection diagnosis and grading." in: Diagnostic pathology, Vol. 8, pp. 168, (2013) (PubMed).

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8 alpha

Background:

Synonyms: T-cell surface glycoprotein CD8 alpha chain, CD8 antigen 32 kDa chain, OX-8 membrane antigen, CD8a

Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Gene ID: 24930

UniProt: P07725

Pathways: TCR Signaling