CCT2 antibody (AA 131-230) (Biotin)

Catalog No. ABIN1390255

This Rabbit Polyclonal antibody specifically detects CCT2 in WB, ELISA, IHC (p), IHC (fro). It exhibits reactivity toward Human, Mouse.

Quick Overview for CCT2 antibody (AA 131-230) (Biotin) (ABIN1390255)

Target: CCT2 (Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCT2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CCT2 Antibody (Biotin)

Binding Specificity: AA 131-230

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CCT2/TCP1 beta

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for CCT2

Target: CCT2 (Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2))

Alternative Name: CCT2/TCP1 beta

Background:

Synonyms: CCT 2, CCT beta, CCT-beta, CCT-2, CCTB, Chaperonin containing t complex polypeptide 1 beta subunit, Chaperonin containing t complex polypeptide 1 subunit 2, Chaperonin containing TCP1 subunit 2, Chaperonin containing TCP1 subunit 2 beta, CTP:phosphocholine cytidylyltransferase 2, MGC142074, MGC142076, MGC94480, PRO1633, T complex protein 1 beta subunit, T complex protein 1 subunit beta, T-complex protein 1 subunit beta, TCP 1 beta, TCP-1-beta, TCPB_HUMAN, 99D8.1.

Background: CCT2 is one of eight largely unrelated subunit proteins found in a protein chaperone complex known as the chaperonin-containing TCP-1 (CCT) or TRiC complex. The CCT complex is an abundanct cytoslic component that is credited with helping newly synthesized polypeptides adopt the correct conformation (1). Proteins that fold and assemble with the help of CCT include the cytoskeletal proteins actin and tubulin as well as up to 15 % of newly synthesized eukaryotic proteins (2). CCT2 is the _-subunit of the chaperone complex and is one of several CCT proteins that exhibit increased expression in response to stress. This implies that the CCT complex helps cells recover from protein damage by assisting in protein folding and assembly (3). CCT subunit levels also change throughout the cell cycle, with lower proteins levels (and reduced chaperone activity) found during induced cell cycle arrest during at M phase (4). Each CCT subunit is thought to perform a specific function during protein folding and assembly (5), CCT2 exhibits both actin and tubulin binding activities (6,3) but the exact molecular function on this subunit remains uncertain.