SH3PXD2A antibody (AA 261-360) (Biotin)

Catalog No. ABIN1392499

This Rabbit Polyclonal antibody specifically detects SH3PXD2A in ELISA, IHC (fro) and IHC (p). It exhibits reactivity toward Rat.

Quick Overview for SH3PXD2A antibody (AA 261-360) (Biotin) (ABIN1392499)

Target: SH3PXD2A (SH3 and PX Domains 2A (SH3PXD2A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3PXD2A antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SH3PXD2A Antibody (Biotin)

Binding Specificity: AA 261-360

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FISH

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for SH3PXD2A

Target: SH3PXD2A (SH3 and PX Domains 2A (SH3PXD2A))

Alternative Name: FISH

Background:

Synonyms: Adapter protein TKS5, Five SH3 domain-containing protein, SH3 and PX domain-containing protein 2A, SH3 multiple domains protein 1, Sh3md1, Sh3pxd2a, SPD2A_HUMAN, TKs5, Tyrosine kinase substrate with five SH3 domains.

Background: Fish, a potential Src substrate, is a broadly expressed adaptor protein containing five SH3 domains and a phox homology (PX) domain (1). The Src family of protein tyrosine kinases act in signal transduction pathways (2-4). Src kinases vary in expression but are strongly regulated in vivo, catalytic activity is repressed by interacting with the SH3 domain (5-7). In Src-transformed fibroblasts and in normal cells treated with certain growth factors fish is tyrosine-phosphorylated (1). Treatment of cells with cytochalasin D results in rapid tyrosine phosphorylation of fish, along with activation of Src (1). Fish is likely to be involved in tyrosine kinase signaling and may have a role in cytoskeletal changes (1).