TBP antibody (AA 201-339) (AbBy Fluor® 647)

Catalog No. ABIN1393194

This anti-TBP antibody is a Rabbit Polyclonal antibody detecting TBP in WB, IF (cc) and IF (p). Suitable for Human, Mouse and Rat.

Quick Overview for TBP antibody (AA 201-339) (AbBy Fluor® 647) (ABIN1393194)

Target: TBP (TATA Box Binding Protein (TBP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBP antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-TBP Antibody (AbBy Fluor® 647)

Binding Specificity: AA 201-339

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TATA binding protein TBP

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TBP

Target: TBP (TATA Box Binding Protein (TBP))

Alternative Name: TFIID/TATA binding protein TBP

Background:

Synonyms: GTF2D, GTF2D1, MGC117320, MGC126054, MGC126055, SCA 17, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, TATA-box-binding protein, TBP, TBP_HUMAN, TF2D, TFIID, Transcription initiation factor TFIID TBP subunit.

Background: In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH, and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Binding of TFIID to the TATA element initiates assembly of the other factors into a pre-initiation complex. The TATA-binding subunit of TFIID (designated TFIIDt or TBP) from higher eukaryotes contains a highly conserved 180 amino acid C-terminal domain.

Pathways: WNT Signaling