JARID2 antibody (AA 301-400) (Biotin)

Catalog No. ABIN1393753

This Rabbit Polyclonal antibody specifically detects JARID2 in WB, ELISA, IHC (p), IHC (fro). It exhibits reactivity toward Human, Rat.

Quick Overview for JARID2 antibody (AA 301-400) (Biotin) (ABIN1393753)

Target: JARID2 (Jumonji, AT Rich Interactive Domain 2 (JARID2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JARID2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-JARID2 Antibody (Biotin)

Binding Specificity: AA 301-400

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Jarid2/Jumonji

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for JARID2

Target: JARID2 (Jumonji, AT Rich Interactive Domain 2 (JARID2))

Alternative Name: Jarid2/Jumonji

Background:

Synonyms: JMJ, Protein Jumonji, Jumonji/ARID domain-containing protein 2, JARID2

Background: Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.

Gene ID: 3720

UniProt: Q92833

Pathways: Chromatin Binding