Radical Fringe (AA 21-120) antibody (AbBy Fluor® 488)

Catalog No. ABIN1394260

This Rabbit Polyclonal antibody specifically detects Radical Fringe in IF (cc) and IF (p). It exhibits reactivity toward Human.

Quick Overview for Radical Fringe (AA 21-120) antibody (AbBy Fluor® 488) (ABIN1394260)

Target: Radical Fringe

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: AbBy Fluor® 488

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details

Binding Specificity: AA 21-120

Predicted Reactivity: Human,Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Radical Fringe

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Radical Fringe

Background:

Synonyms: 3-N-acetylglucosaminyltransferase radical fringe, Beta-1, Beta-1,3-N-acetylglucosaminyltransferase radical fringe, O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, RFNG, RFNG_HUMAN.

Background: Three mammalian fringe family members, Manic, Radical and Lunatic Fringe, have been identified as proteins related to Drosophila Fringe, a protein involved in development. Fringe proteins act upstream of the Notch signaling pathway and are involved in boundary determination during segmentation. Each mammalian Fringe displays different patterns of expression, though all are expressed in the mouse embryo as well as in many adult tissues. Radical Fringe, also known as Beta-1,3-N-acetylglucosaminyltransferase Radical Fringe, is a 331 amino acid single-pass type II membrane protein that localizes to the membrane of the Golgi apparatus. Playing a key role in the development of the limb bud, Radical Fringe transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor. Lunatic Fringe is required for normal somite segmentation and patterning and is thought to be a target of the molecular clock. Manic Fringe, also involved in somatic development, has been shown to render mouse NIH/3T3 cells tumorigenic in SCID mice.