WT1 antibody (AA 301-400) (AbBy Fluor® 350)

Catalog No. ABIN1397778

This anti-WT1 antibody is a Rabbit Polyclonal antibody detecting WT1 in FACS, WB, IF (cc) and IF (p). Suitable for Human, Rat, Mouse and Cow.

Quick Overview for WT1 antibody (AA 301-400) (AbBy Fluor® 350) (ABIN1397778)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human, Rat, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is conjugated to AbBy Fluor® 350

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-WT1 Antibody (AbBy Fluor® 350)

Binding Specificity: AA 301-400

Cross-Reactivity: Cow, Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Wilms Tumor Protein

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: Wilms Tumor Protein

Background:

Synonyms: GUD, AWT1, WAGR, WT33, NPHS4, WIT-2, EWS-WT1, Wilms tumor protein, WT1

Background: Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.

Molecular Weight: 55kDa

Gene ID: 7490

UniProt: P19544

Pathways: Tube Formation