Rubisco antibody (Biotin)

Catalog No. ABIN1397812

This Rabbit Polyclonal antibody specifically detects Rubisco in WB, ELISA, IHC (fro) and IHC (p). It exhibits reactivity toward Arabidopsis thaliana.

Quick Overview for Rubisco antibody (Biotin) (ABIN1397812)

Target: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rubisco antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Rubisco Antibody (Biotin)

Cross-Reactivity: Human, Plant

Cross-Reactivity (Details): Arabidopsis thaliana,

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Arabidopsis thaliana RUBISCO

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Rubisco

Target: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Alternative Name: RUBISCO

Background:

Synonyms: rbcL, RBL_SPIOL, Ribulose bisphosphate carboxylase large chain, RuBisCO large subunit, ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit.

Background: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.Sequence similarities: Belongs to the RuBisCO large chain family. Type I subfamily.Post-translational modifications: The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.

Gene ID: 844754