UBE2A antibody (AA 81-152) (AbBy Fluor® 350)

Catalog No. ABIN1398717

This anti-UBE2A antibody is a Rabbit Polyclonal antibody detecting UBE2A in IF (cc), IF (p). Suitable for Human.

Quick Overview for UBE2A antibody (AA 81-152) (AbBy Fluor® 350) (ABIN1398717)

Target: UBE2A (ube2a) (Ubiquitin-Conjugating Enzyme E2A (ube2a))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2A antibody is conjugated to AbBy Fluor® 350

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-UBE2A Antibody (AbBy Fluor® 350)

Binding Specificity: AA 81-152

Predicted Reactivity: Human,Mouse,Rat,Cow,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UBE2A/RAD6

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UBE2A

Target: UBE2A (ube2a) (Ubiquitin-Conjugating Enzyme E2A (ube2a))

Alternative Name: UBE2A

Background:

Synonyms: BHR6A, hHR6A, HR6A, mHR6A, RAD6 homolog A, RAD6A, RAD6B, UBC-1, UBC2, UBC6, UBCD6, UBE2A, UBE2A_HUMAN, UBE2B, Ubiquitin carrier protein A, Ubiquitin carrier protein, Ubiquitin conjugating enzyme E2 17 kDa, Ubiquitin conjugating enzyme E2 21.5 kDa, Ubiquitin conjugating enzyme E2 A, Ubiquitin protein ligase A, Ubiquitin-conjugating enzyme E2 A, Ubiquitin-conjugating enzyme E2-17 kDa, Ubiquitin-conjugating enzyme E2-21.5 kDa, Ubiquitin-protein ligase A.

Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (Ubiquitin-conjugating enzyme E2 A) and UBE2B (Ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to postreplication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.

Gene ID: 7319