RIT1 antibody (AA 141-219) (Biotin)

Catalog No. ABIN1398823

This Rabbit Polyclonal antibody specifically detects RIT1 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for RIT1 antibody (AA 141-219) (Biotin) (ABIN1398823)

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIT1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RIT1 Antibody (Biotin)

Binding Specificity: AA 141-219

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RIT1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for RIT1

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Alternative Name: RIT1

Background:

Synonyms: GTP binding protein Roc1, GTP-binding protein Rit1, Ras like protein expressed in many tissues, Ras like without CAAX 1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein 1, RIBB, Ric like expressed in many tissues, RIT, RIT1, RIT1_HUMAN, ROC1.

Background: Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation.Neuronal activity dramatically increases the concentration of cytosolic Ca2+, which then serves as a second messenger to direct diverse cellular responses. Calmodulin is a primary mediator of Ca2+ signals in the nervous system. Ric, a protein related to the Ras subfamily of small GTPases, has the ability to bind calmodulin. In addition, two Ras-like human proteins, Rin and Rit (Ric-related gene expressed in many tissues), which are 71 % and 66 % identical to RIC respectively, share related G2 domains with Ric. While most members of the Ras subfamily are plasma membrane-associated and generally require a C-terminal isoprenyl group to bind to the plasma membrane, Rit and Rin lack the recognition signal for C-terminal prenylation. Transiently expressed Rit and Rin are plasma membrane-localized because both proteins contain a C-terminal cluster of basic amino acids, which provides a mechanism for membrane association. Rin binds calmodulin through a C-terminal binding motif. Rit and Ric are widely expressed, whereas expression of Rin is restricted to the neuron system. In conclusion, Rit and Rin define a novel subfamily of Ras-related proteins

Gene ID: 6016

Pathways: Neurotrophin Signaling Pathway