HLA-B27 antibody (AA 81-180) (Biotin)

Catalog No. ABIN1399981

This Rabbit Polyclonal antibody specifically detects HLA-B27 in WB, ELISA, IHC (fro) and IHC (p). It exhibits reactivity toward Human.

Quick Overview for HLA-B27 antibody (AA 81-180) (Biotin) (ABIN1399981)

Target: HLA-B27 (HLA Class I Histocompatibility Antigen, B-27 alpha Chain (HLA-B27))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-B27 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HLA-B27 Antibody (Biotin)

Binding Specificity: AA 81-180

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HLA B27

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for HLA-B27

Target: HLA-B27 (HLA Class I Histocompatibility Antigen, B-27 alpha Chain (HLA-B27))

Alternative Name: HLA B27

Background:

Synonyms: HLA B27 HLA-B27, HLAB, Leukocyte antigen class I B, Lymphocyte antigen, Major histocompatibility complex class I B, SPDA1, 1B27_HUMAN.

Background: Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind peptides and present them to T lymphocytes. Human leukocyte antigens (HLAs) are polymorphic members of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens: class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-B encodes a membrane anchored heavy chain which hetero-dimerizes with a light chain (-2-Microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-B alleles. The HLA-B27 allele appears with increased frequency in uveitis patients.

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response