SASS6 antibody (FITC)

Catalog No. ABIN1401932

The Rabbit Polyclonal anti-SASS6 antibody has been validated for WB and IF (p). It is suitable to detect SASS6 in samples from Human, Mouse and Rat.

Quick Overview for SASS6 antibody (FITC) (ABIN1401932)

Target: SASS6 (Spindle Assembly 6 Homolog (SASS6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SASS6 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-SASS6 Antibody (FITC)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SASS6

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SASS6

Target: SASS6 (Spindle Assembly 6 Homolog (SASS6))

Alternative Name: SASS6

Background:

Synonyms: HsSAS 6, HsSAS6, MGC119440, SAS 6, SAS6, SASS 6, Spindle assembly 6 homolog C. elegans, Spindle assembly 6 homolog, Spindle assembly abnormal protein 6 homolog, PJA2_HUMAN.

Background: SAS-6 (spindle assembly abnormal protein 6 homolog, HsSAS-6) is a 657 amino acid protein encoded by the human gene SAS6. SAS-6 is a component of the centrosome that contains one PISA (present in SAS-6) domain. LK4, SAS-6, CPAP and other centriole related proteins are required at different stages of procentriole formation and were associated with different centriolar structures. SAS-6 associates only transiently with nascent procentrioles, whereas CEP135 and CPAP form a core structure within the proximal lumen of both parental and nascent centrioles. SAS-6 is necessary for procentriole formation in human cell lines and is localized asymmetrically next to the centriole at the onset of procentriole formation. SAS-6 levels oscillate during the cell cycle, it is degraded in mitosis starting at anaphase, and it accumulates again at the end of the following G1 phase. The anaphase-promoting complex targets SAS-6 for degradation by the 26S Proteasome, and a KEN box in the C-terminus of SAS-6 is necessary for its degradation. Increased SAS-6 levels promoted the formation of multiple procentrioles forming next to a single centriole.

Gene ID: 163786