MARCH2 antibody (AbBy Fluor® 555)

Catalog No. ABIN1402133

The Rabbit Polyclonal anti-MARCH2 antibody is suitable to detect MARCH2 in samples from Human, Rat and Mouse. It has been validated for WB and IF (p).

Quick Overview for MARCH2 antibody (AbBy Fluor® 555) (ABIN1402133)

Target: MARCH2 (Membrane-Associated Ring Finger (C3HC4) 2, E3 Ubiquitin Protein Ligase (MARCH2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH2 antibody is conjugated to AbBy Fluor® 555

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-MARCH2 Antibody (AbBy Fluor® 555)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MARCH2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MARCH2

Target: MARCH2 (Membrane-Associated Ring Finger (C3HC4) 2, E3 Ubiquitin Protein Ligase (MARCH2))

Alternative Name: MARCH2

Background:

Synonyms: E3 ubiquitin protein ligase MARCH2, E3 ubiquitin-protein ligase MARCH2, EC 6.3.2.- antibody HSPC240, MARCH II, MARCH-II, march2, MARH2_HUMAN antibody Membrane associated RING CH protein II, Membrane associated ring finger C3HC4 2, Membrane associated RING finger protein 2, Membrane-associated RING finger protein 2, Membrane-associated RING-CH protein II, RING finger protein 172, RNF172.

Background: Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). MARCH2 (membrane-associated ring finger (C3HC4) 2), also known as RNF172 or HSPC240, is a 246 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum and contains one RING-CH-type zinc finger. Expressed in a variety of tissues, MARCH2 functions as an E3 ubiquitin-protein ligase that is thought to mediate the ubiquitination and subsequent degradation of CD71 and B7-2 and may be involved in endosomal protein trafficking.

Gene ID: 51257