CNOT2 antibody (AbBy Fluor® 647)

Catalog No. ABIN1402398

This anti-CNOT2 antibody is a Rabbit Polyclonal antibody detecting CNOT2 in WB and IF (p). Suitable for Human, Mouse and Rat.

Quick Overview for CNOT2 antibody (AbBy Fluor® 647) (ABIN1402398)

Target: CNOT2 (CCR4-NOT Transcription Complex, Subunit 2 (CNOT2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNOT2 antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CNOT2 Antibody (AbBy Fluor® 647)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CNOT2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CNOT2

Target: CNOT2 (CCR4-NOT Transcription Complex, Subunit 2 (CNOT2))

Alternative Name: CNOT2

Background:

Synonyms: CCR4 associated factor 2, CCR4 NOT transcription complex subunit 2, CCR4-associated factor 2, CCR4-NOT transcription complex subunit 2, CDC36, CNOT2, CNOT2_HUMAN, HSPC131, MSTP046, Negative regulator of transcription 2, NOT2 negative regulator of transcription 2 yeast homolog, NOT2, NOT2H.

Background: CNOT2 (CCR4-NOT transcription complex subunit 2) is a ubiquitous protein encoded by the human gene CNOT2. CNOT2 belongs to the CNOT2/3/5 family and is part of the CCR4-NOT complex. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription as well as mRNA degradation. Various subunits (e.g. CNOT1, CNOT2) are involved in influencing nuclear hormone receptor activities. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome. Increased expression of the CNOT2 subunit acts to strongly repress transcription by RNA polymerase II. This repressive effect is mediated by a conserved NOT-Box, which is located at the C-terminus of CNOT2 proteins. Repression by the NOT-Box is sensitive to treatment with the histone deacetylase (HDAC) inhibitor trichostatin A.

Gene ID: 4848

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance