ZWILCH antibody (FITC)

Catalog No. ABIN1404344

The Rabbit Polyclonal anti-ZWILCH antibody has been validated for WB and IF (p). It is suitable to detect ZWILCH in samples from Human, Mouse and Rat.

Quick Overview for ZWILCH antibody (FITC) (ABIN1404344)

Target: ZWILCH (Zwilch, Kinetochore Associated, Homolog (ZWILCH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZWILCH antibody is conjugated to FITC

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ZWILCH Antibody (FITC)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ZWILCH

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ZWILCH

Target: ZWILCH (Zwilch, Kinetochore Associated, Homolog (ZWILCH))

Alternative Name: ZWILCH

Background:

Synonyms: hZwilch, KNTC1AP, MGC111034, Protein zwilch homolog, ZWILC_HUMAN, zwilch,y ZWILCH protein, Zwilch, kinetochore associated, homolog.

Background: Zwilch is the human homolog of the Drosophila Zwilch protein. The Drosophila Zwilch forms a complex with both ROD Rough Deal) and ZWINT (Zeste-White 10, also designated ZW10) proteins. This complex is important for chromosome segregation because it recruits cytoplasmic Dynein to the kinetochore and plays a crucial role in the spindle checkpoint. The role of Zwilch in complex is thought to be evolutionarily conserved because the human homologs of Zwilch, ZWINT and ROD coimmunoprecipitate in a human cell line called HeLa. The human Zwilch, ZWINT and ROD complex localizes to the kinetochores at prometaphase. Mutations were discovered in Zwilch, ZWINT and ROD during a screen for mutations in alleles encoding putative chromosome instability genes in cases of human colorectal cancer. These mutations may contribute in part to the chromosomal instability phenotype of colorectal tumor cells.

Gene ID: 55055

UniProt: Q9H900