EIF5 antibody (AA 331-431) (HRP)

Catalog No. ABIN1406204

The Rabbit Polyclonal anti-EIF5 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect EIF5 in samples from Mouse and Rat.

Quick Overview for EIF5 antibody (AA 331-431) (HRP) (ABIN1406204)

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-EIF5 Antibody (HRP)

Binding Specificity: AA 331-431

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EIF5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EIF5

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Alternative Name: EIF5

Background:

Synonyms: EIF-5, EIF-5A, Eukaryotic translation initiation factor 5, EIF5

Background: Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

Gene ID: 1983

UniProt: P55010