Midline 2 antibody (AA 251-350) (HRP)

Catalog No. ABIN1406744

The Rabbit Polyclonal anti-Midline 2 antibody has been validated for ELISA, IHC (p) and IHC (fro). It is suitable to detect Midline 2 in samples from Human.

Quick Overview for Midline 2 antibody (AA 251-350) (HRP) (ABIN1406744)

Target: Midline 2 (MID2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Midline 2 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Midline 2 Antibody (HRP)

Binding Specificity: AA 251-350

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RNF60/Midline-2

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Midline 2

Target: Midline 2 (MID2)

Alternative Name: RNF60/Midline-2

Background:

Synonyms: FXY2, MID2, Midin-2, Midline 2, Midline defect 2, Midline-2, Probable E3 ubiquitin-protein ligase MID2, RING finger protein 60, Ring finger protein protein 60, RNF60, TRIM1, TRIM1_HUMAN, Tripartite mot protein 1, Tripartite mot-containing protein 1, Tripartite mot-containing protein 1, Midline defect 2, FLJ37715, FLJ41813.

Background: Midline-2 is a 715 amino acid protein encoded by the human gene MID2. Midline-2 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-2 is a cytoplasmic protein found as a homodimer or heterodimer with Midline-1. It also interacts with IGBP1 (Lymphocyte signaling protein A4). Dimerization is mediated by the tripartite motif (RBCC, RING- and B box-type zinc fingers and coiled coil domains) and microtubule association is dependent on the C-terminal B30.2 domain. Midline-2 is expressed at low levels in fetal kidney and lung, and in adult prostate, ovary and small intestine.

Gene ID: 26040