Junctophilin 3 antibody (AA 181-280) (HRP)

Catalog No. ABIN1407062

The Rabbit Polyclonal anti-Junctophilin 3 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect Junctophilin 3 in samples from Human.

Quick Overview for Junctophilin 3 antibody (AA 181-280) (HRP) (ABIN1407062)

Target: Junctophilin 3 (JPH3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Junctophilin 3 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Junctophilin 3 Antibody (HRP)

Binding Specificity: AA 181-280

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Junctophilin 3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Junctophilin 3

Target: Junctophilin 3 (JPH3)

Alternative Name: Junctophilin 3

Background:

Synonyms: JP-3, JP3, JPH3, JPH3_HUMAN, Junctophilin3, Junctophilin type 3, Junctophilin-3, TNRC22, Trinucleotide repeat-containing gene 22 protein.

Background: Junctophilins are components of the junctional complexes between plasma membranes and endoplasmic or sarcoplasmic reticulums present in all excitable cells. Junctophilins contain a cytoplasmic domain which binds to the plasma membrane, as well as an ER/SR membrane spanning hydrophobic C-terminal segment. The three subtypes in this family are Junctophilin-1, -2 and -3. Junctophilin-1 is predominantly expressed in skeletal muscle, but is also expressed at low levels in heart. Junctophilin-2 is expressed in heart and skeletal muscle. Mutant mice lacking the Jph2 gene exhibit embryonic lethality and possess cardiac myocytes that express abnormal calcium transients. Junctophilin-3 is expressed in brain. The JPH3 alternatively spliced exon 2A has been suggested as a site for CTG repeat expansion leading to a Huntington disease-like autosomal dominant disorder.