ZBTB17 antibody (AA 331-430) (HRP)

Catalog No. ABIN1407962

The Rabbit Polyclonal anti-ZBTB17 antibody has been validated for WB, ELISA, IHC (fro) and IHC (p). It is suitable to detect ZBTB17 in samples from Mouse.

Quick Overview for ZBTB17 antibody (AA 331-430) (HRP) (ABIN1407962)

Target: ZBTB17 (Zinc Finger and BTB Domain Containing 17 (ZBTB17))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZBTB17 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ZBTB17 Antibody (HRP)

Binding Specificity: AA 331-430

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Miz1/ZNF60

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ZBTB17

Target: ZBTB17 (Zinc Finger and BTB Domain Containing 17 (ZBTB17))

Alternative Name: Miz1/ZNF60

Background:

Synonyms: Miz-1, Myc-interacting zinc finger protein 1, Myc-interacting zinc finger protein, ZBT17, ZBT17_HUMAN, Zbtb17, Zinc finger and BTB domain containing protein 17, Zinc finger and BTB domain-containing protein 17, Zinc finger protein 151, Zinc finger protein 60, ZNF151, ZNF60.

Background: The Myc family, including c-Myc-, N-Myc- and L-Myc, are nuclear proteins with relatively short half lives that contribute an important role in cellular processes such as proliferation, differentiation, apoptosis and transformation. The c-Myc protein activates transcription as part of a heteromeric complex with a number of interacting partners, including Max and Mxi 1, however the transforming properties of the Myc proto-oncogene are believed to be associated with Myc-mediated transcriptional repression. A POZ domain Zn finger protein, designated Miz-1 for Myc-interacting Zn finger protein-1, is a specific target of Myc-induced gene repression. Miz-1 interacts with Myc, but not Max or other Myc partners, and binding of Myc to Miz-1 requires the helix-loop-helix domain of Myc and a short amphipathic helix located in the carboxy-terminus of Miz-1. Miz-1 associates with DNA elements on the adenovirus major late and cyclin D1 promoters and activates transcription of both promoters. Expression of Miz-1 induces potent growth arrest function, and this latency is reversed by the addition of Myc.

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, ER-Nucleus Signaling