XCL1 antibody (AA 21-114) (HRP)

Catalog No. ABIN1408268

The Rabbit Polyclonal anti-XCL1 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect XCL1 in samples from Human.

Quick Overview for XCL1 antibody (AA 21-114) (HRP) (ABIN1408268)

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XCL1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-XCL1 Antibody (HRP)

Binding Specificity: AA 21-114

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Lymphotactin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for XCL1

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Alternative Name: Lymphotactin/Xcl1

Background:

Synonyms: Lptn, LTN, XCL1 C mot chemokine 1, SCM 1 alpha, ATAC, Chemokine C Mot Ligand 1, Cytokine SCM-1, LPTN, LTN, Lymphotactin, Lymphotaxin, SCM-1-alpha, SCM1, SCYC1, Small inducible cytokine C1, Small Inducible Cytokine Subfamily C Member 1, Small-inducible cytokine C1, XC chemokine ligand 1, Xcl1, XCL1_HUMAN.

Background: Lymphotactin is a chemokine-like molecule produced selectively, if not exclusively, by activated CD8+ T cells (1). Lymphotactin is a C-type member of the chemokine superfamily, but retains only the 2nd and 4th of the four cysteine residues conserved in other chemokines (3,4). In normal tissues, lymphotactin is expressed at high levels in spleen, thymus, small intestine, and peripheral blood leukocytes, as well as at low levels in lung, prostate, and ovary (2). Lymphotactin is chemotactic for CD4+ and CD8+ T cells but not for monocytes, and induces a rise in intracellular calcium in peripheral blood lymphocytes (1). The specific receptor for lymphotactin is XCR1, a member of the G protein-coupled receptor family (5). The gene which encodes lymphotactin maps to human chromosome 1q23 (1,2).

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation