Synaptojanin 1 antibody (AA 481-580) (HRP)

Catalog No. ABIN1408802

The Rabbit Polyclonal anti-Synaptojanin 1 antibody has been validated for ELISA, IHC (fro) and IHC (p). It is suitable to detect Synaptojanin 1 in samples from Rat.

Quick Overview for Synaptojanin 1 antibody (AA 481-580) (HRP) (ABIN1408802)

Target: Synaptojanin 1 (SYNJ1)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptojanin 1 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Synaptojanin 1 Antibody (HRP)

Binding Specificity: AA 481-580

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Synaptojanin/INPP5G

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Synaptojanin 1

Target: Synaptojanin 1 (SYNJ1)

Alternative Name: Synaptojanin 1

Background:

Synonyms: 5-trisphosphate 5-phosphatase 1, Inositol 5' phosphatase, INPP 5G, INPP5G, KIAA0910, Polyphosphoinositide phosphatase, Synaptic inositol 1 4 5 trisphosphate 5 phosphatase 1, Synaptic inositol 145 trisphosphate 5 phosphatase 1, Synaptic inositol-1 antibody Synaptic inositol-145-trisphosphate 5-phosphatase 1, Synaptojanin 1, Synaptojanin 1 polyphosphoinositide phosphatase, Synaptojanin-1, Synaptojanin1, Synj 1, Synj1, SYNJ1_HUMAN.

Background: The inositol polyphosphate 5-phosphatases selectively remove the phosphate from the 5-position of various phosphatidylinositols, which generate second messengers in response to extracellular signals. Synaptojanins are characterized by an N-terminal SAC1-like sequence, a central 5-phosphate domain, and a unique C-terminal sequence and have been shown to use phosphatidylinositol 4,5-bisphosphate as a substrate. Synaptojanins exist as two isoforms, synaptojanin 1 and 2, which differ in the C-terminal domain, and each isoform has multiple variants produced by alternative splicing. Synaptojanin 1 is expressed as two major forms: the shorter is found in brain while the longer is expressed in peripheral tissues. Eight splice variants of synaptojanin 2 have been detected, including a brain specific isoform. Synaptojanins are thought to participate in the endocytosis of synaptic vesicles and the regulation of the actin cytoskeleton.

Gene ID: 8867

Pathways: Inositol Metabolic Process, Synaptic Vesicle Exocytosis