SPEF2 antibody (AA 227-275) (HRP)

Catalog No. ABIN1409456

The Rabbit Polyclonal anti-SPEF2 antibody has been validated for WB, ELISA, IHC (fro) and IHC (p). It is suitable to detect SPEF2 in samples from Human.

Quick Overview for SPEF2 antibody (AA 227-275) (HRP) (ABIN1409456)

Target: SPEF2 (Sperm Flagellar 2 (SPEF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPEF2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SPEF2 Antibody (HRP)

Binding Specificity: AA 227-275

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human KPL2/Sperm Flagellar 2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SPEF2

Target: SPEF2 (Sperm Flagellar 2 (SPEF2))

Alternative Name: KPL2/Sperm Flagellar 2

Background:

Synonyms: KPL2, MGC102842, Protein KPL2, SPEF2, sperm flagellar protein 2, FLJ23164, FLJ23577, FLJ25395, KIAA1770, SPEF2_HUMAN.

Background: Flagella and cillia are both membrane-bound projections from the cell surface that beat in distinctive patterns. Cilia are shorter and usually more profuse than flagella and contain a microtubule cytoskeleton, the ciliary axoneme, surrounded by a ciliary membrane. The ciliary membranes of all cilia hold specific receptors and ion channel proteins that initiate signaling pathways that regulate motility and/or link mechanical or chemical stimuli to intracellular transduction cascades regulating differentiation, migration and cell growth during development and in adulthood. KPL2, also known as SPEF2 (sperm flagellar 2), is a 1,822 amino acid protein that contains a calponin homology domain, three nuclear localization signals, a consensus P-loop and a proline-rich region. Required for correct axoneme develoment, KPL2 is predominantly expressed in cells with cilia or flagella. Four isoforms of KPL2 exists as a result of alternative splicing events.