ARHGEF25/GEFT antibody (AA 251-340) (HRP)

Catalog No. ABIN1411556

The Rabbit Polyclonal anti-ARHGEF25/GEFT antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect ARHGEF25/GEFT in samples from Rat.

Quick Overview for ARHGEF25/GEFT antibody (AA 251-340) (HRP) (ABIN1411556)

Target: ARHGEF25/GEFT (ARHGEF25) (Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF25/GEFT antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ARHGEF25/GEFT Antibody (HRP)

Binding Specificity: AA 251-340

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GEFT

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ARHGEF25/GEFT

Target: ARHGEF25/GEFT (ARHGEF25) (Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25))

Alternative Name: GEFT

Background:

Synonyms: ARHGEF25, ARHGP_HUMAN, GEFT, p63RhoGEF, Guanine nucleotide exchange factor GEFT, p63RhoGEF , RAC/CDC42 exchange factor, Rac/Cdc42/Rho exchange factor GEFT, Rho guanine nucleotide exchange factor 25, RhoA/RAC/CDC42 exchange factor, RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT.

Background: GEFT is a 580 amino acid cytoplasmic protein that is highly expressed in excitable tissues such as brain, heart and muscle, and weakly expressed in small intestine, colon, liver, placenta and lung. GEFT may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. GEFT works as a guanine nucleotide exchange factor for the Rho family of small GTPases and links specifically to G alpha q/11-coupled receptors in Rho A activation. GEFT is an important regulator of processes involved in axon and dendrite formation. Involved in skeletal myogenesis, GEFT seems to be an exchange factor primarily for Rac 1 in neurons. Existing as two alternatively spliced variants, GEFT contains a DH (DBL-homology) domain and a PH domain.