Nfib/Nf1b2 (AA 1-100) antibody (HRP)

Catalog No. ABIN1411880

The Rabbit Polyclonal anti-Nfib/Nf1b2 antibody is suitable to detect Nfib/Nf1b2 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC (fro) and IHC (p).

Quick Overview for Nfib/Nf1b2 (AA 1-100) antibody (HRP) (ABIN1411880)

Target: Nfib/Nf1b2

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 1-100

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow,Sheep,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NFIB/NF1B2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Nfib/Nf1b2

Background:

Synonyms: CCAAT Box Binding Transcription Factor, CCAAT-box-binding transcription factor, CTF, NF-I/B, NF1-B, NF1B, NF1B2, NFI-B, NFI-RED, NFIB, NFIB_HUMAN, NFIB3, Nuclear factor 1 B-type, Nuclear factor 1/B, Nuclear Factor 1B, Nuclear Factor I B, nuclear factor I/B, TGGCA Binding Protein, TGGCA-binding protein.

Background: Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.