SIM2 antibody (AA 321-430) (Cy7)

Catalog No. ABIN1412172

This anti-SIM2 antibody is a Rabbit Polyclonal antibody detecting SIM2 in IF (cc) and IF (p). Suitable for Human.

Quick Overview for SIM2 antibody (AA 321-430) (Cy7) (ABIN1412172)

Target: SIM2 (Single-Minded Homolog 2 (SIM2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIM2 antibody is conjugated to Cy7

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-SIM2 Antibody (Cy7)

Binding Specificity: AA 321-430

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SIM2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SIM2

Target: SIM2 (Single-Minded Homolog 2 (SIM2))

Alternative Name: SIM2

Background:

Synonyms: bHLHe15, Class E basic helix loop helix protein 15, Class E basic helix-loop-helix protein 15, MGC119447, SIM 2, SIM, Sim2, SIM2_HUMAN, Single minded homolog 2 Drosophila, Single minded homolog 2, Single-minded homolog 2, Transcription factor SIM2.

Background: The Per-Arnt-Sim (PAS) domain was identified as a 270 amino acid motif that mediates associations between various PAS family transcription factors. Several PAS domain family members have been identified including AhR, Arnt 1, and single-minded proteins (SIM1 and SIM2). The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator protein 1 (Arnt 1) is required for ligand- dependent nuclear translocation of the Ah receptor and is also necessary for Ah receptor binding to the XRE element. Both SIM1 and SIM2 inhibit AhR/Arnt dimerization, thus inhibiting transcriptional activation. The SIM genes are thought to be involved in the directing and regionalization of tissues during development and the SIM2 gene, which is located on chromosome 21, is a candidate for the gene responsible for Down syndrome.