PPP1R9A antibody (AA 115-165) (Cy3)

Catalog No. ABIN1412391

The Cy3-conjugated Rabbit Polyclonal anti-PPP1R9A antibody (ABIN1412391) specifically detects PPP1R9A in IF (cc) and IF (p). The antibody is reactive with Rat and Mouse samples.

Quick Overview for PPP1R9A antibody (AA 115-165) (Cy3) (ABIN1412391)

Target: PPP1R9A (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 9A (PPP1R9A))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R9A antibody is conjugated to Cy3

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-PPP1R9A Antibody (Cy3)

Binding Specificity: AA 115-165

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Neurabin 1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PPP1R9A

Target: PPP1R9A (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 9A (PPP1R9A))

Alternative Name: Neurabin 1/PPP1R9A

Background:

Synonyms: FLJ20068, KIAA1222, NEB1_HUMAN, Neurabin I, Neurabin-1, Neurabin-I, Neurabin1, Neurabin-1, NeurabinI, Neurabin1, Neural tissue specic F actin binding protein I, Neural tissue-specic F-actin-binding protein I, NRB 1, NRB I, NRB1, NRBI, PPP1R9A, Protein phosphatase 1 regulatory inhibitor subunit 9A, Protein phosphatase 1 regulatory subunit 9A.

Background: Brain-specific neurabin I (neural tissue-specific F-actin binding protein I) is highly concentrated in the synapse of developed neurons, it localizes in the growth cone lamellipodia during neuronal development (1). Suppression of endogenous neurabin in rat hippocampal neurons inhibits neurite formation (1). Neurabin I recruits active PP1 via a PP1-docking sequence, mutation of the PP1-binding motif halts filopodia and restores stress fibers in neurabin I-expressing cells (2,3). Neurabin II (Spinophilin) is ubiquitously expressed but is abundantly expressed in brain (4). Neurabin II localizes to neuronal dentritic spines, which are the specialized protrusions from dendritic shafts that receive most of the excitatory input in the CNS (5). Neurabin II may regulate dendritic spine properties as neurabin II(-) mice have increased spine density during development in vitro and exhibit altered filopodial formation in cultured cells (5). Neurabin may also play a role in glutamatergic transmission as Neurabin II(-) mice exhibit reduced long-term depression and resistance to kainate-induced seizures and neronal apoptosis (5). Neurabin II complexes with the catalytic subunit of protein phosphatase-1 (PP1) in vitro thus modulating the activity of PP1 (4).