CHST1 antibody (AA 251-350) (Cy5.5)

Catalog No. ABIN1414806

This anti-CHST1 antibody is a Rabbit Polyclonal antibody detecting CHST1 in WB, FACS, IF (cc) and IF (p). Suitable for Mouse and Rat.

Quick Overview for CHST1 antibody (AA 251-350) (Cy5.5) (ABIN1414806)

Target: CHST1 (Carbohydrate (Keratan Sulfate Gal-6) Sulfotransferase 1 (CHST1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHST1 antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CHST1 Antibody (Cy5.5)

Binding Specificity: AA 251-350

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Keratan Sulfate

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CHST1

Target: CHST1 (Carbohydrate (Keratan Sulfate Gal-6) Sulfotransferase 1 (CHST1))

Alternative Name: CHST1

Background:

Synonyms: Carbohydrate chondroitin 6 keratan sulfotransferase 1, Carbohydrate chondroitin 6 keratan sulfotransferase, Carbohydrate keratan sulfate Gal 6 sulfotransferase, Carbohydrate keratan sulfate Gal 6 sulfotransferase 1, Carbohydrate sulfotransferase 1, CHST1, EC 2.8.2.21, Galactose N acetylglucosamine N acetylglucosamine 6 O sulfotransferase 1, GST 1 antibody Keratan sulfate Gal 6 sulfotransferase, KS6ST, KSGAL6ST, KSST.

Background: Catalyzes the transfer of sulfate to position 6 of galactose residues of keratan. It has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal galactose residues are sulfated. It also may function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. It participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Aggrecan is the major proteoglycan of human articular cartilage. The core protein is substituted by a number of keratan sulfate and chondroitin sulfate glycosaminoglycan chains. Whereas chondroitin sulfate is widely distributed throughout the body, keratan sulfate is primarily expressed in cartilage (joints, trachea, intervertebral discs) and cornea.

Pathways: Glycosaminoglycan Metabolic Process