LIN28A antibody (AA 65-160) (Cy5)

Catalog No. ABIN1415219

The Rabbit Polyclonal anti-LIN28A antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect LIN28A in samples from Human and Rat.

Quick Overview for LIN28A antibody (AA 65-160) (Cy5) (ABIN1415219)

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIN28A antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-LIN28A Antibody (Cy5)

Binding Specificity: AA 65-160

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Lin-28B

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for LIN28A

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Alternative Name: Lin-28

Background:

Synonyms: Lin28B, CSDD 2, CSDD2, FLJ16517, Lin 28 homolog B C. elegans, Lin 28.2, Lin-28B, LIN28B, LN28B_HUMAN, Protein lin-28 homolog B, Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1.

Background: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Gene ID: 389421, 79727

UniProt: Q6ZN17, Q9H9Z2

Pathways: Stem Cell Maintenance