UBE2U antibody (AA 171-270) (HRP)

Catalog No. ABIN1416646

This Rabbit Polyclonal antibody specifically detects UBE2U in WB, ELISA, IHC (fro) and IHC (p). It exhibits reactivity toward Human.

Quick Overview for UBE2U antibody (AA 171-270) (HRP) (ABIN1416646)

Target: UBE2U (Ubiquitin-Conjugating Enzyme E2U (UBE2U))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2U antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBE2U Antibody (HRP)

Binding Specificity: AA 171-270

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UBE2U

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UBE2U

Target: UBE2U (Ubiquitin-Conjugating Enzyme E2U (UBE2U))

Alternative Name: UBE2U

Background:

Synonyms: UBE2U, UBE2U_HUMAN, Ubiquitin carrier protein U, Ubiquitin-conjugating enzyme E2 U, Ubiquitin-protein ligase U.

Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires ATP-dependent activation of the Ub C-terminus and assembly of multi-Ub chains by a Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2U (ubiquitin-conjugating enzyme E2U) is a 321 amino acid E2 ubiquitin conjugating enzyme that catalyzes the covalent attachment of ubiquitin to other proteins. Existing as two alternatively spliced isoforms, UBE2U is encoded by a gene located on human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8 % of the human genome.

Gene ID: 148581