FBXO15 antibody (AA 101-200) (HRP)

Catalog No. ABIN1416718

This Rabbit Polyclonal antibody specifically detects FBXO15 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Mouse and Rat.

Quick Overview for FBXO15 antibody (AA 101-200) (HRP) (ABIN1416718)

Target: FBXO15 (F-Box Protein 15 (FBXO15))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO15 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FBXO15 Antibody (HRP)

Binding Specificity: AA 101-200

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Fbx15

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FBXO15

Target: FBXO15 (F-Box Protein 15 (FBXO15))

Alternative Name: Fbx15

Background:

Synonyms: F box protein 15, F-box only protein 15, F-box protein 15, Fbxo15, MGC39671, FBX15_HUMAN.

Background: FBXO15, also known as FBX15, is a 434 amino acid protein that contains one C-terminal F-box domain and belongs to the F-box family of proteins. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular processes (including the cell cycle, immune response, signaling cascades and developmental processes) through the targeting of proteins, such as cyclins, cyclin-dependent kinase inhibitors, IkB-a and b-catenin, for degradation by the proteasome after ubiquitination. Via its F-box domain, FBXO15 can directly interact with Skp1 p19 and CUL-1. In addition, FBXO15 is a target of the transcription factor Oct-3/4, however, it does not appear to be essential for early development and fertility.

Gene ID: 201456