CENPK antibody (AA 51-150) (HRP)

Catalog No. ABIN1417084

This Rabbit Polyclonal antibody specifically detects CENPK in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CENPK antibody (AA 51-150) (HRP) (ABIN1417084)

Target: CENPK (Centromere Protein K (CENPK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPK antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CENPK Antibody (HRP)

Binding Specificity: AA 51-150

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FKSG14/Centromere protein K

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CENPK

Target: CENPK (Centromere Protein K (CENPK))

Alternative Name: Centromere protein K

Background:

Synonyms: AF5 alpha, AF5alpha, B130045K24Rik, BB232189, C530004N04Rik, CENP K, CENPK, Centromere protein K, Centromeric protein K, FKSG 14, ICEN 37, ICEN37, Interphase centromere complex protein 37, Leucine zipper protein FKSG14, OTTHUMP00000161888, P33, Protein AF 5alpha, Protein AF5alpha, Solt, Solzt, SoxLZ/Sox6 binding protein Solt, SoxLZ/Sox6 leucine zipper binding protein, CENPK_HUMAN.

Background: FKSG14, also known as CENPK (centromere protein K) is a component of the CENPA-CAD (nucleosome distal) complex. It may be involved in incorporation of CENPA into centromeres and is required for proper kinetochore function, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore. FKSG14 constitutively localized to centromeres throughout the cell cycle. There are 3 isoforms produced by alternative splicing.

Gene ID: 64105