RSAD2 antibody (HRP)

Catalog No. ABIN1418098

This Rabbit Polyclonal antibody specifically detects RSAD2 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RSAD2 antibody (HRP) (ABIN1418098)

Target: RSAD2 (Radical S-Adenosyl Methionine Domain Containing 2 (RSAD2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RSAD2 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RSAD2 Antibody (HRP)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Viperin/RSAD2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RSAD2

Target: RSAD2 (Radical S-Adenosyl Methionine Domain Containing 2 (RSAD2))

Alternative Name: Viperin

Background:

Synonyms: endoplasmic reticulum-associated, interferon-inducible, cig 33, CIG 5, cig-33, CIG-5, CIG33, CIG5, Cytomegalovirus induced gene 5 protein, Cytomegalovirus-induced gene 5 protein, Radical S-adenosyl methionine domain-containing protein 2, RSAD 2, Rsad2, RSAD2_HUMAN, RSDA-2, VIG 1, vig1, Viperin, Virus inhibitory protein, virus inhibitory protein endoplasmic reticulum associated interferon inducible.

Background: RSAD2 is a 361 amino acid protein that is involved in antiviral defense against pathogens such as Hep C, Cytomegalovirus and HIV-1. Localized to the cytosolic side of the endoplasmic reticulum and relocated to the Golgi apparatus upon viral infection, RSAD2 is thought to prevent viral budding by disrupting lipid rafts at the plasma membrane and supporting the Interferon-induced antiviral state of the cell. Additionally, RSAD2 can bind to and inactivate FDPS (an enzyme that is crucial for the synthesis of cholesterol and geranylated and farnesylated proteins), thereby playing a role in lipid synthesis. Overexpression of RSAD2 leads to abnormal lipid accumulation that is associated with atherosclerosis, a chronic inflammatory disease characterized by hardened arteries.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response